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1. Eur J Clin Microbiol Infect Dis. Jun;35(6) doi: /s Epub Mar Since influenza viruses can cause severe illness, timely diagnosis is important for an adequate intervention. The available rapid detection methods either lack. The Solana Influenza A+B Assay is a rapid qualitative in vitro diagnostic test for the detection and differentiation of influenza A and influenza B viral RNA in nasal .

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In addition, the specificity of the assay was assessed using eight reference strains of other respiratory viruses parainfluenza viruses 1 to 3, respiratory syncytial virus Long strain, rhinoviruses 1A and 14, and coronaviruses OC43 and E and 30 combined nose and throat swabs from asymptomatic subjects.

This article has been cited by other articles in PMC. RNA extraction was performed according to the method described by Boom et al. This level of sensitivity correlated with 0. The clinical samples were plotted against the standard curve. Influenza A typically causes the majority of illnesses during flu season.

Although a flu infection is not normally severe, certain populations are at higher risk of developing complications. So chances are, you may benefit at some point in your life from talking…. Rapid diagnostic tests with increased sensitivity are essential for the reliable detection of influenza A and B and enable immediate, effective treatment decisions.

About Abbott is a world leader in rapid diagnostics at the point of care, with a focus on cardio metabolic disease, infectious disease and toxicology. After 2 days of culturing, usually before a cytopathic effect was noticed, rapid antigen testing was performed by immunofluorescence with commercial monoclonal antibodies for influenza viruses A and B shell vial culturing.


Read More – Investors Back. Some of these specimens were obtained at regional general practices participating in a study to evaluate the efficacy of influenza vaccination.

Support Center Support Center. However, in this study, the transport conditions did not affect the sensitivity of conventional culturing, although the number of tested clinical specimens was small. Lower Your Risk of Getting the Flu. Besides being rapid, this method also has the advantage of a standardized protocol that can be applied easily to other respiratory viruses; the TaqMan PCR can be performed under uniform amplification conditions, thereby allowing the use of target-specific primer and probe sets.

Alere i Influenza A & B – Alere is now Abbott

It typically spreads through respiratory droplets when a person who has the flu sneezes or coughs. Therefore, rapid diagnosis is important not only for timely therapeutic intervention but also for the identification of a beginning influenza outbreak. Influenza virus infection is a highly contagious respiratory disease that can spread easily and that is responsible for considerable morbidity and mortality each year.

Application of a fluorogenic PCR assay for typing and subtyping of influenza viruses influehza respiratory samples. The reporter dye FAM signal was measured against the internal reference dye ROX signal to normalize for non-PCR-related fluorescence fluctuations occurring from well to well. Unbound material was removed by several washing steps. Real time quantitative PCR.

For epidemiological reasons, it is important to type and subtype influenza virus strains. What Ingredients Are in the Flu Shot?

How Are Influenza A and B Different?

Both influenza A and influenza B are extremely contagious. Robinson JL, et al. Moreover, since the concentrations of viable virus can decline rapidly after the first few days of the infection, the virus can become undetectable by culturing in the later course of the infection 7.


Abstract Since influenza viruses can cause severe illness, timely diagnosis is important for an adequate intervention. Menu Location Alere is now Abbott. Even trying to cut back on the sweet stuff…. July Picard C, et al. Antiviral medications are most effective when started within the first 48 hours of your illness. Lower Your Risk of Getting the Flu Learn about strategies to keep from getting the flu, and how to recover quickly if you do get the bug.

These subtypes are based off of the combination of two proteins on the viral surface: Rapid 3-in-1 detection of main gastroenteritis viruses.

Longitudinal follow-up of six patients with either influenza virus A patients 3 to 6 or virus B infection patients 1 and 2. The threshold cycle represented the refraction cycle number at which a positive amplification reaction was measured and was set at 10 times the standard deviation of the mean baseline emission calculated for PCR cycles 3 to Read on to learn more.

How are influenza A and influenza B different? A standard curve could be generated with the multiplex TaqMan PCR, creating a quantitative format for the assay. Multiplex detection of four pathogenic retroviruses using molecular beacons. In addition, the procedure is less complicated than other RT-PCR methods, and the chances of contamination are minimized because there is no post-PCR processing of the samples. Open in a separate window.

Facts, Statistics, iinfluenza You The flu, or influenza, is a contagious respiratory illness caused by viruses that infect the nose, throat, and sometimes the lungs.