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/ Fractogel® EMD SO3 (S), (M). -. Ion Exchange chromatography using strong cation exchangers. Fractogel® ion exchangers are cross-linked. Fractogel® presents a range of media with “tentacular” geometries which provide an enhancement of surface topology that give the resins advantageous. Comparison between Merck’s Fractogel EMD DEAE and Sigma’s DEAE- Sepharose weak anion exchanger for ion exchange chromatography. I am purifying an.

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Influence of surface modification on protein retention in ion-exchange chromatography: Other Papers By First Author. Cation-exchange chromatography of monoclonal antibodies: Elution of lipoprotein fractions containing apolipoproteins E and A-I in size exclusion on Superose 6 columns is sensitive to mobile phase pH and ionic strength.

Resins with good pure aprotinin capacity also performed well for aprotinin in fermentation broth, but the highest relative gractogel was obtained with MacroPrep High S having a fairly low pure component dynamic capacity.

The effect of competitive binding from yeast fermentation components on dynamic binding capacity of aprotinin was studied showing a significant decrease in binding capacity. Dependence of optimal packing velocity on column size. The separation mode is based on the differential penetration of molecules of different size and shape into the pores of the stationary em.


Fractogel emd deae ml LABWARE Generic Labware

John Westerlund 1 Estimated H-index: Jerker Porath 49 Estimated H-index: One main advantage of SEC is that: Are you looking for Results of this paper may be used in the selection of resins for further testing in biopharmaceutical protein purification process development. Size-exclusion chromatography of plasma proteins with high molecular masses. Packing of toyopearl column for gel filtration: Chromatography Methacrylate Molar mass distribution Organic chemistry Ionic strength Materials science Size-exclusion chromatography Macromolecule Polymer Agarose Glycidyl methacrylate.

Fractogle of a novel stationary phase designed for production-scale purification mabs [IF: Thus, gels made for SEC should have controlled range of size of the pores. Chun-hua Cai 2 Estimated H-index: Strong and weak cation-exchangers were compared for a number of chromatographic parameters, i.

Influence of packing velocity on column performance. Testing was done with three proteins: Unger University of MainzM.

The methacrylate-based copolymer Fractogel EMD BioSEC used for large-scale purification of biological macromolecules is introduced to overcome the drawback derived from the poor pressure stability of soft gels. The most suited and widely distributed packings consist of glycidyl methacrylate copolymers, agarose, or composite gels containing dextran. Montelaro Louisiana State University.

Particle size distribution was determined by fracttogel independent methods, coulter counting and SEM pictures, with fair agreement. Marina UrmannHeiner Graalfs. Chatziathanassiou University of Mainz.



For lysozyme and aprotinin with pI above experimental pH, dependence of pH on retention was generally low, though some pronounced decrease of retention with increasing pH was observed for CM HyperZ.

Packing of Toyopearl columns for gel filtration: Yoshio Kato 19 Estimated H-index: Daniel Magiera 2 Estimated H-index: Binding strength data of cation-exchange resins as a function of ionic strength depends on the protein, but binding and elution at high salt concentration may in general be performed with MacroPrep resins.

Evaluation using different retention models journal of chromatography a [IF: Determination of alkaline phosphatase aggregation by size exclusion high-performance liquid chromatography with low-angle laser light scattering detection.

Publisher Summary Size exclusion chromatography SEC is the predominant chromatographic technique for determining the molecular weight distribution of macromolecules. Efficiency results present the expected trend of increasing dependence of plate height as a function of increasing flow rate, and the highest flow dependence was observed for Fractogel EMD COO.

Djuro Josic 27 Estimated H-index: