ASTM E2149 PDF
ASTM E Antimicrobial Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents Under Dynamic Contact Conditions. ASTM E Standard Test Method for Determining the Antimicrobial Activity of Immobilized Antimicrobial Agents under Dynamic Contact Conditions. The test.
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Referenced Documents purchase separately The documents listed below are referenced within the subject standard but are not provided as part of the standard. Historical Version s – view previous versions of standard. If an antimicrobial agent that is shown to be removed from the surface by Section 13 is utilized in this test methodology, controls must be included such that appropriate neutralization steps are including during recovery and enumeration. Presence of residual antimicrobial activity indicates the presence of a leaching type of antimicrobial agent.
Ast, Documents purchase separately The documents listed below are referenced within the subject standard but are not provided as part of the standard.
It is for this reason that the use of the term leaching throughout this document is limited to only the testing conditions described herein. A single cookie will be used in your browser to remember your preference not to be tracked. Summary of Test Method: Note 1 — Stresses may include laundry, wear and abrasion, radiation and steam sterilization, UV exposure, solvent manipulation, temperature susceptibility, or similar physical or chemical manipulation.
These difficulties include ensuring contact of e22149 to treated surface as in AATCCflexibility of retrieval at different contact times, use of inappropriately applied static conditions as in AATCCsensitivity, and reproducibility.
ASTM E – Microbe Investigations (MIS)
This dynamic shake flask test was developed for routine quality control and screening tests in order to overcome difficulties in using classical antimicrobial test methods to evaluate substrate-bound antimicrobials. Following exposure, a sample of the test astj suspension is removed quantitatively assayed for survivors.
The ASTM E Standard Test Method is used to evaluate the antimicrobial activity of substrate-bound, non-leaching, antimicrobial-treated samples under dynamic contact conditions. It has been developed for routine quality control and screening tests and is used to evaluate substrate bound antimicrobials. This standard does not purport to astmm all of the safety concerns, if any, associated with its use.
Link to Active This link e21499 always route to the current Active version of the standard. Summary of Test In this method, treated test samples are placed in a laboratory flask containing a dilute suspension of test organism, commonly Escherichia coli.
This test ensures good contact between bacteria and the treated fibre by constant agitation of the test specimen in a bacterial suspension during the test period 1hour. Maria Curry ext Wrist Action Shaker. One-hour contact time in a buffer solution allows for metabolic stasis in the population.
This test method is designed to evaluate the resistance of specimens 2e149 with a non-leaching antimicrobial agent to the growth of microbes under dynamic contact conditions.
ASTM E2149 Antimicrobial Activity
It is the responsibility of astmm user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Organisms typically used in this method: For example, water soluble antimicrobials will be prone to removal from the test surface using the method described in Section 13 but insoluble compounds will not.
This test method standardizes both the growth conditions of the challenge species and substrate contact times to reduce the variability associated with growth phase of the microorganism. The number of viable organisms from the suspension is determined and asstm percent reduction is calculated by comparing retrievals from appropriate controls.
To determine if a compound is immobilized in all conditions or during the end use of the product additional testing may be required.
Work Item s – proposed revisions of this standard. The antimicrobial activity of a substrate-bound, non-leaching antimicrobial agent is dependent upon direct contact of microbes with the active chemical agent. Test Package Request Form. Active view current version of standard.
All the samples are shaken in dynamic shake flasks in e22149 wrist action shaker to ensure good contact between the bacteria and the treated fibre, fabric, or other substrate by constant agitation of the test specimen in a bacterial suspension during the test period.
ASTM E – Method Determining the Antimicrobial Activity
These difficulties include ensuring contact of inoculum to treated surface as in AATCCflexibility of retrieval at different contact times, use of inappropriately adtm static conditions as in AATCCsensitivity, and reproducibility.
This test method ensures good contact between the bacteria and the treated fiber, fabric, or other substrate, by constant agitation of the test specimen in a challenge suspension during the test period. Log in Log out Edit. Materials evaluated by this method may be comprised of fabric, paper, powder or other solid materials. The presence of a leaching antimicrobial is determined post-test. This method is generally used to substantiate antimicrobial properties of treated specimens for non-public health related claims.
Immobilized antimicrobial agents, such as surface bonded materials, are not free to diffuse into their environment under normal conditions of use. This test method is only intended to determine efficacy as described in 4. We use this information to improve and customize your browsing xstm and for analytics and metrics about our visitors — both on this website and other media. No regulated reduction limits currently exist for general antimicrobial claims made using this method.